The tuning frequency is determined by the port volume, and internal enclosure volume. Ao yes to question 1.
Question 2 is often debated, but I have never heard a difference with the port on a different side than the woofer. Just as long as you have a couple of inches between the port openeing and the nearest wall you should be fine.
Don't forget to subtract the woofer and the port displacement from the internal volume.
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